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The Cipher Genetic Analysis System detects mutations and polymorphisms in 4 different ways: SSCP, TTGE, CDGE or DGGE. Destabilization of nucleic acid complexes can be accomplished using a uniform solvent concentration (Urea/Formamide) with an increasing temperature gradient (TTGE) or separation can be achieved by varying the solvent concentration at a constant temperature (DGGE). Both techniques rely on establishing a range of either solvent or temperature in which the target fragments will undergo conformational transition (melt) Single Strand Conformational Polymorphisms (SSCP) reveals differences in electrophoretic mobility between normal and mutant strands of DNA. Includes: electrophoresis tank, EPS-300-II Mini-Power Supply, mini-peristaltic pump, 2 single cassettes, heater/stirrer/buffer cycler with temperature ramping capability, gradient maker, 4 combs (2ea 1 Well and 2 each 16 Well combs), 4 sets of spacers (2 sets for vertical DGGE & TTGE and 2 sets for perpendicular DGGE), 2 glass plate sets, 4 Gel Wrap? gaskets, clamps, glass safety cover, buffer siphon pump, large diameter cooling coil, power leads, and gel tape. Call C.B.S. Scientific for more ordering information and refer to Product Literature on Home page for a more detailed description.
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